74104 qiagen protocol pdf

These products arenotintendedforthediagnosis,prevention,ortreatmentofadisease. Qiaquick pcr purification kit protocols using a microcentrifuge 19 using a vacuum manifold 21 qiaquick nucleotide removal kit protocol 23 qiaquick gel extraction kit protocols using a microcentrifuge 25 using a vacuum manifold 27 troubleshooting guide 30 appendix. Isolate a suitable piece of tissue and place in a uvcrosslinked 1. The qiagen bench guide is designed to help you with your laboratory work. Background information, protocols, hints, and tips are provided for purification and analysis of plasmid dna, genomic dna, rna, and proteins, as well as recipes for buffers and solutions. Our advanced, highquality products and services ensure success from sample to result. This protocol is designed for purification of total dna from grampositive bacteria. For more information, please consult the appropriate safety data sheets. Pdf qiagen genomic dna handbook leia torres academia. The protocols differ primarily in the lysis and homogenization of the sample and in the adjustment of the conditions for binding rna to the rneasy membrane. Qiaquick gel extraction kit protocol using a microcentrifuge. Qiagen rneasy micro kit could be substituted for the qiaamp viral rna mini kit.

The qiacube is preinstalled with protocols for purification of plasmid dna, genomic dna, rna, viral nucleic acids, and proteins, plus dna and rna cleanup. One volume of 70% ethanol was mixed with each lysate, and. L qiagen protease or proteinase k into the bottom of a 1. Australia qiagen pty ltd po box 25 clifton hill victoria 3068 abn 75 072 382 944 orders 0394893666 fax 0394893888 technical 1800243066 canada qiagen inc. Free qiagen kit offer at university of rochester stockroom. Qiagen plasmid kits are intended for molecular biology applications.

For purelink kits, an input of 150 mg was also tested. Other than expressly stated licenses, qiagen makes no warranty that this kit andor its uses do not infringe the rights of thirdparties. Tested with rneasy micro kit qiagen protocol, in the developmental stage, and northern blot of high quality and growth conditions. Qiagen sample and assay technologies qiagen is the leading provider of innovative sample and assay technologies, enabling the isolation and detection of contents of any biological sample. In this handbook, different protocols are provided for different starting materials. Qiagen distributors please see the last page for contact information for your local qiagen distributor. Start protocol rnalater rna stabilization reagent, rnalater tissueprotect tubes, and. Homogenize you tissue check out the how to homogenize tissues for details.

Rneasy mini handbook 092010 5 kit contents rneasy mini kit 50 250 catalog no. Once the sample is bound to the membrane, the protocols are similar see flowchart, next page. If you have any questions or experience any difficulties regarding the qiaamp minelute virus spin kit or qiagen products in general, please do not hesitate to contact us. Safety information when working with chemicals, always wear a suitable lab coat, disposable gloves and protective goggles. Should any product fail to perform satisfactorily due to any reason other than misuse, qiagen will replace it free of charge or refund the purchase price. Forceps are to be sterilized in fine science tools heat block at. Qiagen guarantees the performance of all products in the manner described in our product literature. Qiagen protocols are optimized for use with yeast cultures grown in standard ypd media see page 57 to a cell density of approximately 3 x 10 8 cellsml.

Total rna was extracted with the rneasy mini kit qiagen, cat. If using 150500 mg of tissue, use half the volumes used below consult the qiagen rneasy handbook for further details. Forefront tower ii 1, kachidoki 3 chome chuoku, tokyo 1040054. These are available online in convenient and compact pdf format. Rneasy mini kit, qiagen, pn 74104 polya mrna isolation oligotex direct mrna kit isolation of mrna from whole cells, qiagen, pn 72012, 72022, or 72041 oligotex mrna kit isolation of mrna from total rna, qiagen, pn 70022, 70042, or 70061 qiashredder, qiagen, pn 79654 required only for use with qiagen oligotex direct kit. For more information, additional and more detailed protocols, and safety information, please refer. Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol. Up to 400 mg agarose can be processed per spin column. In addition to products for preparing rna, qiagen offers a range of other. These protocols have not been thoroughly tested or optimized by qiagen. The range of protocols available is continually expanding, and additional qiagen protocols can. On rna with rneasy micro kit is the kit delivers highly reproducible ct values were from small amounts of genomic and rna. Rna, proteins, dyes, and lowmolecularweight impurities are removed by a mediumsalt wash. Protocol qiaquick spin handbook 032001 23 qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer.

The purchaser must determine the suitability of the product for its particular use. These are available online in convenient and compact pdf format at. Rneasy plant mini kit for purification of total rna from plants and filamentous fungi. Part i laboratory preparation and storage of a salivaoragenerna sample purification steps notes 1. Wheeler lab protocols dna extraction qiagen dneasy kit. For purification of up to 100 micro g total rna from cells, tissues, and yeast.

Jun 02, 2014 kit contents rneasy mini kit 50 250 catalog no. For more information, additional and more detailed protocols, and safety. Qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. Quickstartprotocol rneasy mini kit, part 1 january 2011. We recommend all users of qiagen products to adhere to the nih guidelines that have. For purification of total rna from animal cells, animal tissues, bacteria, and yeast, and for. With the rneasy mini kit, total rna is easily purified from 10 to 1 x 10 7 animal or human cells, 0. Now until april 30th at the university of rochester. Other than expressly stated licenses, qiagen makes no warranty that this kit andor its uses do not infringe the rights of. Qiagen customers are a major source of infor mation regarding advanced or specialized. A variety of special application protocols is also available.

Kit contents qiagen pcr cloning kit qiagen pcr cloning kit 10 40 catalog no. Australia qiagen pty ltd po box 25 clifton hill victoria 3068 abn 75 072 382 944 orders 0394893666 fax 0394893888 technical 0394893666 canada qiagen inc. The dilution factor should then be used in calculating the number of cells per ml. Qiagen neither guarantees them nor warrants that they do not infringe the rights of thirdparties. Via grosio, 1010 20151 milano orders 0233430411 fax 0233430426 technical 0233430414 japan qiagen k. Oragenerna purification protocol using the qiagen rneasy. Rna concentration was measured spectrophotometrically at 260 nm nanodrop8000, thermo fishcer and rna purity determined by the a260a280, a230260 ranges and spectral patterns. Qiavac vacuum manifolds 33 references 36 ordering information 37. Rna isolation protocol using the qiagen rneasy mini kit osski. The brief version of the procedure is below for reference. Qiagen plasmid plasmid kit mega 5 mega 25 giga 5 buffer set catalog no. All due care and attention should be exercised in the handling of the products. Step 6 from booklet is an additional spin for 15 sec, with 45 l tris.

For more information and protocols, please contact qiagen technical service at support. Rneasy protect mini kit for immediate stabilization of rna in harvested animal tissues and subsequent total rna purification. Rneasy mini kit for purification of total rna from animal cells, animal tissues, bacteria, and yeast, and for rna cleanup. Quickstart protocol april 2016 rneasy mini kit, part 2 qiagen. Arrange appropriately labeled 50 ml conical tubes with 15 ml of trizol or buffer rlt with 2mercaptoethanol in each on ice. Qiagen plasmid purification handbook harvard university. Com rnasefree dnase set for dnase treatment with qiagen or preanalytix rna purification kits kit contents rnasefree dnase set 50 catalog no. Rneasy micro handbook 042003 5 all due care and attention should be exercised in the handling of the products. For cleanup of other enzymatic reactions, follow the protocol as described for pcr samples or use the minelute reaction cleanup kit. Organized so that you can find the information you need. Qiaamp dna mini kit and qiaamp dna blood mini kit handbook.